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|---|---|---|---|---|---|---|---|
| 品牌 | abinScience | ||||||
| 种属反应性 | Human | ||||||
| 应用 | ELISA, FCM, WB | ||||||
| 同种型 | IgG2a, kappa | ||||||
| 克隆号 | SAA0527 | ||||||
| 靶标 | Complement factor H, CFH, H factor 1, HF1, HF, HF2 | ||||||
| 内毒素水平 | Please contact with the lab for this information. | ||||||
| 纯度 | >95% as determined by SDS-PAGE. | ||||||
| 纯化方式 | Protein A/G purified from cell culture supernatant. | ||||||
| Accession号 | P08603 | ||||||
| RRID | Anti-Human CFH Antibody (SAA0527) (abinScience Cat# HY505013, RRID:AB_3726045) | ||||||
| 状态 | Liquid | ||||||
| 保存溶液 | 0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
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| 产品使用信息 |
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| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| Note | For research use only. | ||||||
| Isotype Control | Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023) |

Flow-cytometry using anti-human CFH antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CFH antibody monoclonal antibody (Catalog # HY505013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for Anti-Human CFH Antibody (SAA0527).

Flow-cytometry using PE anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CFH monoclonal antibody (Catalog: HY505013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using FITC anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CFH monoclonal antibody (Catalog: HY505013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using APC anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CFH monoclonal antibody (Catalog: HY505013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.











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