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Anti-Human DRD1/AGIA Recombinant Antibody (SAA0783) (HB176013)

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规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

  • 图片

  • 参考文献

  • 产品说明书

概述
货号HB176013
品牌abinScience
描述
Anti-Human DRD1/AGIA Recombinant Antibody (SAA0783) (HB176013) is a mouse monoclonal antibody detecting DRD1/AGIA in ELISA, IF, IP, SPR, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
种属反应性Human
应用ELISA, IF, IP, SPR, WB
宿主Mouse
克隆类型Monoclonal
同种型IgG2a, kappa
克隆号SAA0783
靶标Dopamine D1 receptor, DRD1, D(1A) dopamine receptor
内毒素水平< 10 EU/mg as determined by LAL method.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P21728
状态Liquid
保存溶液 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

D(1A) dopamine receptor (DRD1/AGIA) is a ~49 kDa protein. Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. Forms heterotetramers with DRD3 to potentiate beta-arrestin recruitment and mediate locomotor activity.

NoteFor research use only.
Isotype ControlMouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)
图片
  • Western blot

    Western blot analysis was performed using anti-DRR1 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant human DRR1 (Catalog No: HB176012)
    Lane 2: negative control

  • SDS-PAGE

    SDS-PAGE for Anti-Human DRD1/AGIA Recombinant Antibody (SAA0783)

  • Immunofluorescence

    Immunofluorescence staining of SHSY-5Y with Mouse monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the cytoplasm.

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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