Anti-Human APLNR Recombinant Nanobody (SAA1317) (HW667013)

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50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HW667013

品牌

abinScience

描述

Anti-Human APLNR Nanobody (SAA1317) [SAA1317] (HW667013) is a alpaca monoclonal antibody detecting APLNR in ELISA, FCM, SPR. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

种属反应性

Human

应用

ELISA, FCM, SPR

宿主

Alpaca

克隆类型

Monoclonal

同种型

VHH-8His-Cys-tag

克隆号

SAA1317

靶标

G-protein coupled receptor HG11, Apelin receptor, AGTRL1, APJ, Angiotensin receptor-like 1, APLNR, G-protein coupled receptor APJ

内毒素水平

< 10 EU/mg as determined by LAL method.

纯度

>95% as determined by SDS-PAGE.

纯化方式

Purified by Nickel column.

Accession号

P35414

状态

Liquid

保存溶液

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Apelin receptor (APLNR/APJ) is a ~42 kDa protein. G protein-coupled receptor for peptide hormones apelin (APLN) and apelin receptor early endogenous ligand (APELA/ELA), that plays a role in the regulation of normal cardiovascular function and fluid homeostasis. When acting as apelin receptor, activates both G(i) protein pathway that inhibits adenylate cyclase activity, and the beta-arrestin pathway that promotes internalization of the receptor. APLNR/APJ also functions as mechanoreceptor that is activated by pathological stimuli in a G-protein-independent fashion to induce beta-arrestin signaling, hence eliciting cardiac hypertrophy. However, the presence of apelin ligand blunts cardiac hypertrophic induction from APLNR/APJ on response to pathological stimuli. Plays a key role in early development such as gastrulation, blood vessels formation and heart morphogenesis by acting as a APELA receptor.

1. Cayabyab, M. et al. (2000) Journal of virology 74, 11972-6. PMID: 11090199

2. Scimia, MC. et al. (2012) Nature 488, 394-8. PMID: 22810587

3. Wang, Z. et al. (2015) Scientific reports 5, 8170. PMID: 25639753

4. Yang, P. et al. (2017) Circulation 135, 1160-1173. PMID: 28137936

5. Yue, Y. et al. (2022) Nature structural & molecular biology 29, 688-697. PMID: 35817871

6. Wang, WW. et al. (2024) Cell 187, 1460-1475.e20. PMID: 38428423

Note

For research use only.

图片

SDS-PAGE

SDS-PAGE for Anti-Human APLNR Nanobody (SAA1317)

参考文献

产品说明书

Anti-Human APLNR Recombinant Nanobody (SAA1317).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human APLNR Recombinant Nanobody (SAA1317) (HW667013)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human APLNR Recombinant Nanobody (SAA1317) (HW667013)

概述

图片

参考文献

产品说明书

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