Anti-Human VEGFR2/KDR/Flk-1 Recombinant Nanobody (SAA1230) (HW338023)

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规格：

50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HW338023

品牌

abinScience

描述

Anti-Human CD309/KDR/VEGFR-2 Nanobody (SAA1230) [SAA1230] (HW338023) is a alpaca monoclonal antibody detecting VEGFR-2 in ELISA, FCM, SPR. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

种属反应性

Human

应用

ELISA, FCM, SPR

宿主

Alpaca

克隆类型

Monoclonal

同种型

VHH-8His-Cys-tag

克隆号

SAA1230

经测试应用

靶标

CD309, EC:2.7.10.1, FLK-1, FLK1, Fetal liver kinase 1, KDR, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, VEGFR-2, VEGFR2, Vascular endothelial growth factor receptor 2

内毒素水平

< 10 EU/mg as determined by LAL method.

纯度

>95% as determined by SDS-PAGE.

纯化方式

Purified by Nickel column.

Accession号

P35968

状态

Liquid

保存溶液

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Vascular endothelial growth factor receptor 2 (CD309/VEGFR-2) is a ~151 kDa protein. Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. CD309 is the therapeutic target of ramucirumab (Cyramza).

Note

For research use only.

图片

SDS-PAGE

SDS-PAGE for Anti-Human CD309/KDR/VEGFR-2 Nanobody (SAA1230)
Bioactivity

Detects recombinant CD309 (Catalog No: HW338011) in indirect ELISA.
Immunofluorescence

Immunofluorescence staining of Hepg2 with Alpaca monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the Cytoplasm ，Nucleus ，Cell membrane,Secreted .

参考文献

产品说明书

Anti-Human VEGFR2/KDR/Flk-1 Recombinant Nanobody (SAA1230).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human VEGFR2/KDR/Flk-1 Recombinant Nanobody (SAA1230) (HW338023)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human VEGFR2/KDR/Flk-1 Recombinant Nanobody (SAA1230) (HW338023)

概述

图片

参考文献

产品说明书

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