Anti-Listeria monocytogenes serotype 1/2a InlB/Internalin B Recombinant Nanobody (SAA0997) (JN937023)

价格:

规格：

50ug
100ug
1mg

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

JN937023

品牌

abinScience

描述

Anti-Listeria monocytogenes serotype 1/2a InlB/Internalin B Nanobody (SAA0997) [SAA0997] (JN937023) is a alpaca monoclonal antibody detecting Internalin B in ELISA.

种属反应性

Listeria monocytogenes

应用

ELISA

宿主

Alpaca

同种型

VHH-8His-Cys-tag

克隆号

SAA0997

克隆类型

Monoclonal

靶标

Internalin B, InlB, Invasion protein InlB, inlB

内毒素水平

Please contact with the lab for this information.

纯度

>95% as determined by SDS-PAGE.

纯化方式

Purified by Nickel column.

Accession号

P0DQD3

状态

Liquid

保存溶液

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Internalin B (InlB) is a ~71 kDa protein. Mediates the entry of L.monocytogenes into normally non-phagocytic mammalian host cells (Probable). Its host receptor is hepatocyte growth factor receptor (HGF receptor, a tyrosine kinase, MET) which is tyrosine-phosphorylated in response to InlB in human, green monkey, mouse and dog cell lines. Downstream adapter proteins GAB1 and CBL are phosphorylated in response to InlB, which also causes cell colony scattering. InlB binding to mammalian cells is saturable and inhibited by EDTA; InlB-coated beads can be taken up by host cells. Complement component 1 Q subcomponent-binding protein (gC1q-R, C1QBP) might act as an InlB receptor, leading to activation of PI3-kinase in green monkey cells.

1. Shen, Y. et al. (2000) Cell 103, 501-10. PMID: 11081636

2. Braun, L. et al. (1997) Molecular microbiology 25, 285-94. PMID: 9282740

3. Banerjee, M. et al. (2004) Molecular microbiology 52, 257-71. PMID: 15049825

4. Braun, L. et al. (2000) The EMBO journal 19, 1458-66. PMID: 10747014

5. Marino, M. et al. (2002) The EMBO journal 21, 5623-34. PMID: 12411480

Note

For research use only.

图片

SDS-PAGE

SDS-PAGE for Anti-Listeria monocytogenes serotype 1/2a InlB/Internalin B Nanobody (SAA0997)

参考文献

产品说明书

Anti-Listeria monocytogenes serotype 1/2a InlB/Internalin B Recombinant Nanobody (SAA0997).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Listeria monocytogenes serotype 1/2a InlB/Internalin B Recombinant Nanobody (SAA0997) (JN937023)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Listeria monocytogenes serotype 1/2a InlB/Internalin B Recombinant Nanobody (SAA0997) (JN937023)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价