Anti-Clostridium botulinum BoNT/B Recombinant Nanobody (SAA0977) (JN959013)

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50ug
100ug
1mg

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概述
图片
参考文献
产品说明书

概述

货号

JN959013

品牌

abinScience

描述

Anti-Clostridium botulinum BoNT/B Nanobody (SAA0977) [SAA0977] (JN959013) is a alpaca monoclonal antibody detecting BoNT/B in ELISA.

种属反应性

Clostridium botulinum

应用

ELISA

宿主

Alpaca

同种型

VHH-8His-Cys-tag

克隆号

SAA0977

克隆类型

Monoclonal

靶标

Botulinum neurotoxin type B, BoNT/B, Bontoxilysin-B, Botulinum neurotoxin B light chain, LC, 3.4.24.69, Botulinum neurotoxin B heavy chain, HC, botB

内毒素水平

Please contact with the lab for this information.

纯度

>95% as determined by SDS-PAGE.

纯化方式

Purified by Nickel column.

Accession号

P10844

状态

Liquid

保存溶液

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Botulinum neurotoxin type B (BoNT) is a ~150 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin B which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins found in synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol.

1. Chai, Q. et al. (2006) Nature 444, 1096-100. PMID: 17167418

2. Jin, R. et al. (2006) Nature 444, 1092-5. PMID: 17167421

3. Rummel, A. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 359-64. PMID: 17185412

4. Berntsson, RP. et al. (2013) Nature communications 4, 2058. PMID: 23807078

5. Dong, M. et al. (2003) The Journal of cell biology 162, 1293-303. PMID: 14504267

6. Hoch, DH. et al. (1985) Proceedings of the National Academy of Sciences of the United States of America 82, 1692-6. PMID: 3856850

7. Nishiki, T. et al. (1994) The Journal of biological chemistry 269, 10498-503. PMID: 8144634

10. Sun, S. et al. (2012) Biochemistry 51, 5655-62. PMID: 22720883

Note

For research use only.

图片

SDS-PAGE

SDS-PAGE for Anti-Clostridium botulinum BoNT/B Nanobody (SAA0977)

参考文献

产品说明书

Anti-Clostridium botulinum BoNT/B Recombinant Nanobody (SAA0977).pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Clostridium botulinum BoNT/B Recombinant Nanobody (SAA0977) (JN959013)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Clostridium botulinum BoNT/B Recombinant Nanobody (SAA0977) (JN959013)

概述

图片

参考文献

产品说明书

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