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Anti-E.coli lexA/LexA repressor Recombinant Nanobody (SAA1458) (JN826013)

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  • 100ug
  • 1mg
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概述
货号JN826013
品牌abinScience
描述
Anti-E.coli lexA/LexA repressor Nanobody (SAA1458) [SAA1458] (JN826013) is a alpaca monoclonal antibody detecting LexA repressor in ELISA.
种属反应性Escherichia coli
应用ELISA
宿主Alpaca
同种型VHH-8His-Cys-tag
克隆号SAA1458
克隆类型Monoclonal
靶标LexA repressor, lexA
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Purified by Nickel column.
Accession号Q1R3P3
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

LexA repressor is a ~22 kDa protein. Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.

NoteFor research use only.
图片
  • SDS-PAGE

    SDS-PAGE for Anti-E.coli lexA/LexA repressor Nanobody (SAA1458)

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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