首页 > 产品中心 > 抗体 > 一抗

Anti-ZEBOV NP/Nucleoprotein Recombinant Antibody (KZ51) (VK518023)

价格:
规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

  • 图片

  • 参考文献

  • 产品说明书

概述
货号VK518023
品牌abinScience
种属反应性Zaire ebolavirus (ZEBOV) (Zaire Ebola virus)
应用ELISA, IF
同种型IgG
克隆号KZ51
靶标NP, Nucleoprotein, Nucleocapsid protein
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P18272
RRIDAnti-ZEBOV NP/Nucleoprotein Antibody (KZ51) (abinScience Cat# VK518023, RRID:AB_3727247)
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Nucleoprotein (NP) is a ~83 kDa protein. Oligomerizes into helical capsid to encapsidate the viral genome, protecting it from nucleases and the cellular innate immune response. VP35 binds to and stabilizes monomeric NP, keeping it soluble. Upon virus replication, NP is recruited to bind cooperatively viral genomic RNA and VP35 is released. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. The nucleocapsid is helical with a pitch of 10.81 NP per turn and a diameter of about 22nm.

1. Johnson, RF. et al. (2006) Virology journal 3, 31. PMID: 16719918
2. Leung, DW. et al. (2015) Cell reports 11, 376-89. PMID: 25865894
3. Kirchdoerfer, RN. et al. (2015) Cell reports 12, 140-149. PMID: 26119732
4. Sugita, Y. et al. (2018) Nature 563, 137-140. PMID: 30333622
5. Wan, W. et al. (2017) Nature 551, 394-397. PMID: 29144446
6. Beniac, DR. et al. (2012) PloS one 7, e29608. PMID: 22247782
7. Kirchdoerfer, RN. et al. (2016) PLoS pathogens 12, e1005937. PMID: 27755595
NoteFor research use only.
图片
  • SDS-PAGE

    SDS-PAGE for Anti-ZEBOV NP/Nucleoprotein Antibody (KZ51)

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

相关产品推荐