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Anti-Human IL3 Recombinant Antibody (SAA1520) (HY262013)

价格:
规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

  • 图片

  • 参考文献

  • 产品说明书

概述
货号HY262013
品牌abinScience
种属反应性Human
应用ELISA, WB
同种型IgG2a, kappa
克隆号SAA1520
靶标IL3, Hematopoietic growth factor, Multipotential colony-stimulating factor, IL-3, Mast cell growth factor, Interleukin-3, P-cell-stimulating factor, MCGF
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P08700
RRIDAnti-Human IL3 Antibody (SAA1520) (abinScience Cat# HY262013, RRID:AB_3727281)
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Interleukin-3 (IL3) is a ~17 kDa protein. Cytokine secreted predominantly by activated T-lymphocytes as well as mast cells and osteoblastic cells that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells. Also stimulates mature basophils, eosinophils, and monocytes to become functionally activated. In addition, plays an important role in neural cell proliferation and survival. Participates as well in bone homeostasis and inhibits osteoclast differentiation by preventing NF-kappa-B nuclear translocation and activation. Mechanistically, exerts its biological effects through a receptor composed of IL3RA subunit and a signal transducing subunit IL3RB.

1. Guba, SC. et al. (1989) The Journal of clinical investigation 84, 1701-6. PMID: 2556442
2. Okayama, Y. et al. (1993) Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 23, 901-10. PMID: 10779277
3. Hong, L. et al. (2020) Cytokine 136, 155268. PMID: 32889153
4. Luo, XJ. et al. (2012) PloS one 7, e50375. PMID: 23226269
5. Khapli, SM. et al. (2003) Journal of immunology (Baltimore, Md. : 1950) 171, 142-51. PMID: 12816992
6. Broughton, SE. et al. (2018) Nature communications 9, 386. PMID: 29374162
7. López, C. et al. (2017) Journal of cellular biochemistry 118, 1330-1340. PMID: 27862234
NoteFor research use only.
图片
  • SDS-PAGE

    SDS-PAGE for Anti-Human IL3 Antibody (SAA1520)

  • Bioactivity

    Detects recombinant human IL3 (Catalog No: HY262012) in indirect ELISA.

  • Bioactivity

    Detects recombinant human IL3 (Catalog No: HY262011) in indirect ELISA.

  • Western blot

    Western blot analysis was performed using anti-IL3 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant human IL3 (Catalog No: HY262011)

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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