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Anti-Human RNF43 Recombinant Antibody (SAA1758) (HP618013)

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规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

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  • 参考文献

  • 产品说明书

概述
货号HP618013
品牌abinScience
种属反应性Human
应用ELISA, WB
宿主Mouse
克隆类型Monoclonal
同种型IgG2a, kappa
克隆号SAA1758
靶标E3 ubiquitin-protein ligase RNF43, RING finger protein 43, RING-type E3 ubiquitin transferase RNF43, RNF43
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号Q68DV7
RRIDAnti-Human RNF43 Antibody (SAA1758) (abinScience Cat# HP618013, RRID:AB_3727519)
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

E3 ubiquitin-protein ligase RNF43 is a ~85 kDa protein. E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Along with RSPO2 and ZNRF3, constitutes a master switch that governs limb specification.

1. Sugiura, T. et al. (2008) Experimental cell research 314, 1519-28. PMID: 18313049
2. Hao, HX. et al. (2012) Nature 485, 195-200. PMID: 22575959
3. Koo, BK. et al. (2012) Nature 488, 665-9. PMID: 22895187
NoteFor research use only.
Isotype ControlMouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)
图片
  • Western blot

    Western blot analysis was performed using anti-mouse RNF43 monoclonal antibody at 1ug/mL on various samples.
    Lane 1: ATG11865 200ng
    Lane 2: negative control

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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