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Anti-Human DDX4/Vasa Recombinant Antibody (SAA1970) (HV699013)

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规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

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  • 参考文献

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概述
货号HV699013
品牌abinScience
种属反应性Human
应用ELISA
同种型IgG2a, kappa
克隆号SAA1970
靶标DDX4, VASA, Vasa homolog, DEAD box protein 4, Probable ATP-dependent RNA helicase DDX4
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号Q9NQI0
RRID

Anti-Human DDX4/Vasa Antibody (SAA1970) (abinScience Cat# HV699013, RRID:AB_3727734)
_x005F_x000D_

状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Probable ATP-dependent RNA helicase DDX4 (Vasa) is a ~79 kDa protein. ATP-dependent RNA helicase required during spermatogenesis. Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs.

1. Castrillon, DH. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 9585-90. PMID: 10920202
2. Li, HJ. et al. (2010) Chinese medical journal 123, 2879-82. PMID: 21034600
NoteFor research use only.
图片
  • SDS-PAGE

    SDS-PAGE for Anti-Human DDX4/Vasa Antibody (SAA1970)

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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