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Anti-Neisseria meningitidis NHBA/Dextranase Recombinant Antibody (5H2) (JN891033)

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规格:
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  • 100ug
  • 1mg
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概述
货号JN891033
品牌abinScience
种属反应性Neisseria meningitidis
应用ELISA, SPR
同种型IgG1, kappa
克隆号5H2
靶标nhba, gna2132, Heparin binding protein, Neisserial heparin binding antigen, Genome-derived neisserial antigen 2132, Putative lipoprotein GNA2132, NHBA
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号Q9JPP1
RRID

Anti-Neisseria meningitidis NHBA/Dextranase Antibody (5H2) (abinScience Cat# JN891033, RRID:AB_3727794)
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状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Neisserial heparin binding antigen (NHBA/Dextranase) is a ~44 kDa protein. A major human immunogenic protein detected in patients recovering from meningitidis, where it induces bactericidal antibodies. Binds human cells, heparin and heparan sulfate proteoglycan in vitro via the Arg-rich motif. Heparin-binding to this protein protects bacteria against killing by bactericidal antibodies (serum killing). The bacteria binds a number of human extracellular sialyated and/or sulfated glycans via this protein, including chondroitin sulfate, heparin and ganglioside GT3. Binds DNA non-specifically.

1. Maritan, M. et al. (2018) PloS one 13, e0201922. PMID: 30133484
NoteFor research use only.
图片
  • Bioactivity

    Detects Neisseria meningitidis NHBA/Dextranase in indirect ELISAs.

  • SDS-PAGE

    SDS-PAGE for Anti-Neisseria meningitidis NHBA/Dextranase Antibody (5H2).

  • Western blot

    Western blot analysis was performed using anti-Neisseria meningitidis NHBA/Dextranase monoclonal antibody at 1µg/ml on various samples.
    Lane 1: recombinant Neisseria meningitidis NHBA (Catalog No: JN891012)
    Lane 2: negative control

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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