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Anti-Human IL-12/IL-23 p40 Recombinant Antibody (SAA2017) (HB769023)

价格:
规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

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  • 参考文献

  • 产品说明书

概述
货号HB769023
品牌abinScience
种属反应性Human
应用ELISA, FCM, WB
同种型IgG1, lambda
克隆号SAA2017
经测试应用WB, FCM
靶标IL-12 subunit p40, IL12B, Interleukin-12 subunit beta, IL-12B, CLMF p40, NKSF2, NK cell stimulatory factor chain 2, Cytotoxic lymphocyte maturation factor 40 kDa subunit
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P29460
RRID

Anti-Human IL12B/IL-12 p40/NKSF2 Antibody (SAA2017) (abinScience Cat# HB769023, RRID:AB_3727929)
_x005F_x005F_x000D_

状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Interleukin-12 subunit beta (IL12B/IL-12 p40) is a ~37 kDa protein. Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.

NoteFor research use only.
Isotype ControlHuman IgG1, lambda Isotype Control antibody (HyHEL-10) (HP248107)
图片
  • Bioactivity

    SEC-HPLC detection for Anti-Human IL12B/IL-12 p40/NKSF2 Antibody (ABS0390).

  • Flow Cytometry

    Flow-cytometry using anti-human IL12B antibody.IL12B Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL12B antibody monoclonal antibody (Catalog # HB769023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • SDS-PAGE

    SDS-PAGE for Anti-Human IL12B/IL-12 p40/NKSF2 Antibody (SAA2017).

  • Western blot

    Western blot analysis was performed using anti-IL12B/IL-12 p40/NKSF2 monoclonal antibody at 1μg/ml on various samples.
    Lane 1: recombinant human IL12B/IL-12 p40/NKSF2 (Catalog No: HB769021), 50ng
    Lane 2: recombinant human IL12B/IL-12 p40/NKSF2 (Catalog No: HB769021), 30ng
    Lane 3: recombinant human IL12B/IL-12 p40/NKSF2 (Catalog No: HB769021), 20ng

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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