| 货号 | HB769023 | ||||||
|---|---|---|---|---|---|---|---|
| 品牌 | abinScience | ||||||
| 种属反应性 | Human | ||||||
| 应用 | ELISA, FCM, WB | ||||||
| 宿主 | Human | ||||||
| 同种型 | IgG1, lambda | ||||||
| 克隆号 | SAA2017 | ||||||
| 克隆类型 | Monoclonal | ||||||
| 靶标 | IL-12 subunit p40, IL12B, Interleukin-12 subunit beta, IL-12B, CLMF p40, NKSF2, NK cell stimulatory factor chain 2, Cytotoxic lymphocyte maturation factor 40 kDa subunit | ||||||
| 内毒素水平 | Please contact with the lab for this information. | ||||||
| 纯度 | >95% as determined by SDS-PAGE. | ||||||
| 纯化方式 | Protein A/G purified from cell culture supernatant. | ||||||
| Accession号 | P29460 | ||||||
| 状态 | Liquid | ||||||
| 保存溶液 | 0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
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| 产品使用信息 |
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| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| Note | For research use only. |
SEC-HPLC detection for Anti-Human IL12B/IL-12 p40/NKSF2 Antibody (ABS0390).
Flow-cytometry using anti-human IL12B antibody.IL12B Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL12B antibody monoclonal antibody (Catalog # HB769023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for Anti-Human IL12B/IL-12 p40/NKSF2 Antibody (SAA2017).
Western blot analysis was performed using anti-IL12B/IL-12 p40/NKSF2 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human IL12B/IL-12 p40/NKSF2 (Catalog No: HB769021), 50ng
Lane 2: recombinant human IL12B/IL-12 p40/NKSF2 (Catalog No: HB769021), 30ng
Lane 3: recombinant human IL12B/IL-12 p40/NKSF2 (Catalog No: HB769021), 20ng
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