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Anti-Human CTSB Recombinant Antibody (2A2) (HY436013)

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规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

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  • 参考文献

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概述
货号HY436013
品牌abinScience
种属反应性Human
应用WB
同种型IgG2a, kappa
克隆号2A2
靶标APP secretase, CPSB, Cathepsin B, Cathepsin B1, CTSB, APPS
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P07858
RRID

Anti-Human CTSB Antibody (2A2) (abinScience Cat# HY436013, RRID:AB_3728963)
_x005F_x005F_x000D_

状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Cathepsin B (CTSB) is a ~37 kDa protein. Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen. Has also been implicated in tumor invasion and metastasis.

1. Guo, R. et al. (2002) Biochemical and biophysical research communications 297, 38-45. PMID: 12220505
2. Ritonja, A. et al. (1985) FEBS letters 181, 169-72. PMID: 3972105
NoteFor research use only.
Isotype ControlMouse IgG2a Recombinant Isotype Control Antibody (S43.10) (MF533307)
图片
  • SDS-PAGE

    SDS-PAGE for Anti-Human CTSB Antibody (2A2).

  • Flow CytoMetry

    Flow-cytometry using FITC anti-human CTSB antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CTSB monoclonal antibody (Catalog: HY436013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

  • Western blot

    Western blot analysis was performed using anti-CTSB monoclonal antibody at 1μg/ml on various samples.
    Lane 1: recombinant human CTSB (Catalog No: HY436012), 50ng
    Lane 2: recombinant human CTSB (Catalog No: HY436012), 30ng
    Lane 3: recombinant human CTSB (Catalog No: HY436012), 20ng

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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