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Anti-Human CTSB Antibody (2A2) (HY436013)

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规格:
  • 50ug
  • 100ug
  • 1mg
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概述
货号HY436013
品牌abinScience
种属反应性Human
应用WB
宿主Mouse
同种型IgG2a, kappa
克隆号2A2
克隆类型Monoclonal
靶标APP secretase, CPSB, Cathepsin B, Cathepsin B1, CTSB, APPS
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P07858
状态Liquid
保存溶液 0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
NoteFor research use only.
图片
  • SDS-PAGE

    SDS-PAGE for Anti-Human CTSB Antibody (2A2).

  • Flow CytoMetry

    Flow-cytometry using FITC anti-human CTSB antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CTSB monoclonal antibody (Catalog: HY436013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

  • Western blot

    Western blot analysis was performed using anti-CTSB monoclonal antibody at 1μg/ml on various samples.
    Lane 1: recombinant human CTSB (Catalog No: HY436012), 50ng
    Lane 2: recombinant human CTSB (Catalog No: HY436012), 30ng
    Lane 3: recombinant human CTSB (Catalog No: HY436012), 20ng

参考文献

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