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Anti-Escherichia coli O157:H7 shiga toxin type 1 B/stx1B Recombinant Antibody (5-5B) (JN072013)

价格:
规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

  • 图片

  • 参考文献

  • 产品说明书

概述
货号JN072013
品牌abinScience
种属反应性Escherichia coli
应用ELISA, FCM, WB
同种型IgG2a
克隆号5-5B
靶标stx1, stx1B, Shiga toxin 1 B subunit, Shiga toxin I B subunit, Escherichia coli O157:H7
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号Q7BQ98, Q32GM0, P69178, P69179, Q779K3
RRID

Anti-Escherichia coli O157:H7 shiga toxin type 1 B/stx1B Antibody (5-5B) (abinScience Cat# JN072013, RRID:AB_3729026)
_x005F_x000D_

状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Shiga toxin subunit B (shiga toxin type 1B/stx1B) is a ~9 kDa protein. The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.

NoteFor research use only.
图片
  • Bioactivity

    SEC-HPLC detection for Anti-Escherichia coli O157:H7 shiga toxin type 1 B/stx1B Antibody (5-5B).

  • Western blot

    Western blot analysis was performed using anti-stx1B monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant E. coli stx1B (Catalog No: JN072012)
    Lane 2: negative control

  • Bioactivity

    Detects recombinant E. coli shiga toxin type 1 B/stx1B (Catalog No: JN072012) in indirect ELISA

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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