Anti-Human Gal1/LGALS1 Recombinant Antibody (8A12) (HY272023)

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规格：

50ug
100ug
1mg

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概述
图片
参考文献
产品说明书

HY272023

abinScience

种属反应性

Human

ELISA, FCM

IgG2a, kappa

HLBP14, Lactose-binding lectin 1, LGALS1, HBL, HPL, Beta-galactoside-binding lectin L-14-I, Gal-1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, Galaptin, Galectin-1, 14 kDa laminin-binding protein, 14 kDa lectin

内毒素水平

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Accession号

P09382

Liquid

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Galectin-1 (Gal-1) is a ~14 kDa protein. Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. Plays a negative role in Th17 cell differentiation via activation of the receptor CD69.

1. de la Fuente, H. et al. (2014) Molecular and cellular biology 34, 2479-87. PMID: 24752896

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Bioactivity

Detects Human Gal1/LGALS1 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human Galectin-1 antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-1 antibody monoclonal antibody (Catalog # HY272023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human Galectin-1 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-1 antibody monoclonal antibody (Catalog # HY272023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human Gal1/LGALS1 Antibody (8A12).
Flow cytometry

Flow-cytometry using anti-human Galectin-1 antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-1 monoclonal antibody (Catalog HY272023, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

产品说明书

Anti-Human Gal1/LGALS1 Recombinant Antibody (8A12).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

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Anti-Human Gal1/LGALS1 Recombinant Antibody (8A12) (HY272023)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human Gal1/LGALS1 Recombinant Antibody (8A12) (HY272023)

概述

图片

参考文献

产品说明书

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