Anti-Human Fc gamma RIII/CD16 Recombinant Nanobody (SAA2149) (HY386023)
概述
图片
参考文献
产品说明书
概述
货号
HY386023
品牌
abinScience
描述
Anti-Human CD16/FcgR3 Nanobody (SAA2149) [SAA2149] (HY386023) is a alpaca monoclonal antibody detecting CD16-I in Blocking, ELISA. Suitable for Human.
种属反应性
Human
应用
Blocking, ELISA, WB
宿主
Alpaca
同种型
VHH-8His-Cys-tag
克隆号
SAA2149
克隆类型
Monoclonal
经测试应用
WB
靶标
Low affinity immunoglobulin gamma Fc region receptor III-B, Fc-gamma RIII-beta, CD16-I, Fc-gamma RIII, Fc-gamma RIIIb, FcRIII, FcRIIIb, FcR-10, IgG Fc receptor III-1, CD16b, FCGR3B, CD16B, FCG3, FCGR3, IGFR3, Low affinity immunoglobulin gamma Fc region receptor III-A, IgG Fc receptor III-A, CD16-II, CD16a antigen, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcgammaRIIIA, FcR-10, IgG Fc receptor III-2, CD16a, FCGR3A, CD16A, FCG3, FCGR3, IGFR3
内毒素水平
Please contact with the lab for this information.
纯度
>95% as determined by SDS-PAGE.
纯化方式
Purified by Nickel column.
Accession号
P08637, O75015
状态
Liquid
保存溶液
0.01M PBS pH 7.4
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:2000-1:20000
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Low affinity immunoglobulin gamma Fc region receptor III-A (CD16/FcgR3) is a ~29 kDa protein. Receptor for the invariable Fc fragment of immunoglobulin gamma (IgG). Optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, triggers lysis of antibody-coated cells, a process known as antibody-dependent cellular cytotoxicity (ADCC). Does not bind free monomeric IgG, thus avoiding inappropriate effector cell activation in the absence of antigenic trigger. Mediates IgG effector functions on natural killer (NK) cells. Binds antigen-IgG complexes generated upon infection and triggers NK cell-dependent cytokine production and degranulation to limit viral load and propagation.
1. Hezareh, M. et al. (2001) Journal of virology 75, 12161-8. PMID: 11711607
2. Ferrara, C. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 12669-74. PMID: 21768335
3. Mizushima, T. et al. (2011) Genes to cells : devoted to molecular & cellular mechanisms 16, 1071-80. PMID: 22023369
4. DiLillo, DJ. et al. (2014) Nature medicine 20, 143-51. PMID: 24412922
5. Lee, J. et al. (2015) Immunity 42, 431-42. PMID: 25786175
6. Jing, Y. et al. (2015) PloS one 10, e0121788. PMID: 25816339
7. Blázquez-Moreno, A. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E5645-E5654. PMID: 28652325
8. de Haas, M. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 2948-55. PMID: 8609432
SDS-PAGE for Anti-Human CD16/FcgR3 Nanobody (SAA2149).
Bioactivity
Detects recombinant CD16b (Catalog No: HF886011) in indirect ELISA.
Western blot
Western blot analysis was performed using anti-CD16b monoclonal antibody at 1ug/mL on various samples. Lane 1: recombinant human CD16b (Catalog No: HF886011)
