Anti-Human BRD4 IP Recombinant Antibody (SAA0192) (HF757013)

价格:

规格：

50ug
100ug
1mg

数量：

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概述
图片
参考文献
产品说明书

HF757013

abinScience

种属反应性

Human

ELISA, IF, IP, IP-MS

Mouse

Monoclonal

IgG2a

SAA0192

Protein HUNK1, Bromodomain-containing protein 4, BRD4, HUNK1

内毒素水平

Please contact with the lab for this information.

>95% as determined by SDS-PAGE.

Protein A/G purified from cell culture supernatant.

Accession号

O60885

Anti-Human BRD4 IP Antibody (SAA0192) (abinScience Cat# HF757013, RRID:AB_3729726)

Liquid

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Bromodomain-containing protein 4 (BRD4) is a ~152 kDa protein. Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters. Also recruits P-TEFb complex to distal enhancers, so called anti-pause enhancers in collaboration with JMJD6. BRD4 and JMJD6 are required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II.

1. Filippakopoulos, P. et al. (2010) Nature 468, 1067-73. PMID: 20871596

2. Zhang, W. et al. (2012) The Journal of biological chemistry 287, 43137-55. PMID: 23086925

3. Wu, SY. et al. (2013) Molecular cell 49, 843-57. PMID: 23317504

4. Konuma, T. et al. (2017) Scientific reports 7, 16272. PMID: 29176719

5. Olley, G. et al. (2018) Nature genetics 50, 329-332. PMID: 29379197

6. Wang, R. et al. (2012) The Journal of biological chemistry 287, 10738-52. PMID: 22334664

7. Patel, MC. et al. (2013) Molecular and cellular biology 33, 2497-507. PMID: 23589332

8. Jang, MK. et al. (2005) Molecular cell 19, 523-34. PMID: 16109376

9. Yang, Z. et al. (2005) Molecular cell 19, 535-45. PMID: 16109377

For research use only.

SDS-PAGE

SDS-PAGE for Anti-Human BRD4 IP Antibody (SAA0192).

产品说明书

Anti-Human BRD4 IP Recombinant Antibody (SAA0192).pdf

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

分子量 *

g/mol

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

C₁ (起始浓度)

V₁ (起始体积)

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human BRD4 IP Recombinant Antibody (SAA0192) (HF757013)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human BRD4 IP Recombinant Antibody (SAA0192) (HF757013)

概述

图片

参考文献

产品说明书

相关产品推荐

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