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Anti-Human SMYD3 IP Recombinant Antibody (SAA0230) (HP320013)

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规格:
  • 50ug
  • 100ug
  • 1mg
数量:
  • 概述

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  • 参考文献

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概述
货号HP320013
品牌abinScience
种属反应性Human
应用ELISA, FCM, IF, IP
同种型IgG1
克隆号SAA0230
靶标Zinc finger MYND domain-containing protein 1, SET and MYND domain-containing protein 3, SMYD3, ZMYND1, Histone-lysine N-methyltransferase SMYD3, ZNFN3A1
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
纯化方式Protein A/G purified from cell culture supernatant.
Accession号Q9H7B4
RRIDAnti-Human SMYD3 IP Antibody (SAA0230) (abinScience Cat# HP320013, RRID:AB_3729764)
状态Liquid
保存溶液 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:2000-1:20000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Histone-lysine N-methyltransferase SMYD3 is a ~49 kDa protein. Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Also methylates 'Lys-5' of histone H4. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.

1. Hamamoto, R. et al. (2004) Nature cell biology 6, 731-40. PMID: 15235609
2. Van Aller, GS. et al. (2012) Epigenetics 7, 340-3. PMID: 22419068
NoteFor research use only.
Isotype ControlMouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)
图片
  • Flow Cytometry

    Flow-cytometry using anti-human SMYD3 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human SMYD3 antibody monoclonal antibody (Catalog # HP320013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • SDS-PAGE

    SDS-PAGE for Anti-Human SMYD3 IP Antibody (SAA0230).

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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