Anti-VDAC1 Polyclonal Antibody (HB031014) is a rabbit polyclonal antibody detecting VDAC1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
种属反应性
Human, Mouse, Rat
应用
ELISA, IHC, WB
宿主
Rabbit
同种型
IgG
克隆类型
Polyclonal
免疫原
E. coli - derived recombinant Human VDAC1 (Met1-Ala283).
靶标
Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, hVDAC1, Porin 31HL, Porin 31HM, Voltage-dependent anion-selective channel protein 1, VDAC1, VDAC-1, VDAC
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Non-selective voltage-gated ion channel VDAC1 is a ~30 kDa protein. Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol.
1. Stadtmüller, U. et al. (1999) Biological chemistry 380, 1461-6. PMID: 10661876
2. Thinnes, FP. et al. (2001) Pflugers Archiv : European journal of physiology 443 Suppl 1, S111-6. PMID: 11845315
3. Hiller, S. et al. (2008) Science (New York, N.Y.) 321, 1206-10. PMID: 18755977
4. Marginedas-Freixa, I. et al. (2018) Scientific reports 8, 11384. PMID: 30061676
5. Blachly-Dyson, E. et al. (1993) The Journal of biological chemistry 268, 1835-41. PMID: 8420959
6. Dadsena, S. et al. (2019) Nature communications 10, 1832. PMID: 31015432
7. Ham, SJ. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 4281-4291. PMID: 32047033
10. Chen, Y. et al. (2010) Biochemical and biophysical research communications 394, 798-803. PMID: 20230784
Note
For research use only.
图片
Western blot
Western blot analysis was performed using anti-VDAC1 polyclonal antibody at 1µg/ml on various samples. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: Mouse brain lysate Lane 4: Rat brain lysate Lane 5: Mouse heart lysate Lane 6: Rat heart lysate
