Anti-Histone H3 Polyclonal Antibody (HV603014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HV603014

品牌

abinScience

描述

Anti-Histone H3 Polyclonal Antibody (HV603014) is a rabbit polyclonal antibody detecting Histone H3/o in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Acropora formosa, and Aspergillus niger.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human H3C15 (Met1-Ala136).

靶标

HIST2H3A, H3C15, Histone H3/o, H3-clustered histone 14, H3-clustered histone 15, H3-clustered histone 13, H3F2, H3FM, Histone H3/m, HIST2H3D, HIST2H3C, Histone H3.2

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q71DI3

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

重悬

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

产品使用信息

应用方法	稀释比例
ELISA	1:2000-1:20000
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Histone H3.2 (H3C15) is a ~15 kDa protein. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-H3C15 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: A549 cell lysate
Lane 6: K562 cell lysate
Lane 7: Mouse spleen lysate

参考文献

产品说明书

Anti-Histone H3 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Histone H3 Polyclonal Antibody (HV603014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Histone H3 Polyclonal Antibody (HV603014)

概述

图片

参考文献

产品说明书

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