Anti-Human IKBKG Polyclonal Antibody (HA521014) is a rabbit polyclonal antibody detecting IKBKG in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, Sus scrofa, and Human.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
种属反应性
Human
应用
ELISA, IHC, WB
宿主
Rabbit
克隆类型
Polyclonal
同种型
IgG
免疫原
E. coli - derived recombinant Human IKBKG (Met1-Glu223).
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
NF-kappa-B essential modulator (IKBKG) is a ~48 kDa protein. Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin plays a key role in IKK activation by multiple signaling receptor pathways. Can recognize and bind both 'Lys-63'-linked and linear polyubiquitin upon cell stimulation, with a much higher affinity for linear polyubiquitin. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Essential for viral activation of IRF3.
1. Zhou, H. et al. (2004) Nature 427, 167-71. PMID: 14695475
2. Arimoto, K. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 15856-61. PMID: 20724660
3. Zotti, T. et al. (2011) The Journal of biological chemistry 286, 22924-33. PMID: 21518757
