| 背景 | Outer kinetochore KNL1 complex subunit ZWINT is a ~31 kDa protein. Acts as a component of the outer kinetochore KNL1 complex that serves as a docking point for spindle assembly checkpoint components and mediates microtubule-kinetochore interactions. Kinetochores, consisting of a centromere-associated inner segment and a microtubule-contacting outer segment, play a crucial role in chromosome segregation by mediating the physical connection between centromeric DNA and spindle microtubules. The outer kinetochore is made up of the ten-subunit KMN network, comprising the MIS12, NDC80 and KNL1 complexes, and auxiliary microtubule-associated components; together they connect the outer kinetochore with the inner kinetochore, bind microtubules, and mediate interactions with mitotic checkpoint proteins that delay anaphase until chromosomes are bioriented on the spindle. Targets the RZZ complex to the kinetochore at prometaphase. Recruits MAD2L1 to the kinetochore, but is not required for BUB1B localization. 2. Wang, H. et al. (2004) The Journal of biological chemistry 279, 54590-8. PMID: 154858113. Kops, GJ. et al. (2005) The Journal of cell biology 169, 49-60. PMID: 158241315. Petrovic, A. et al. (2014) Molecular cell 53, 591-605. PMID: 245303017. Yatskevich, S. et al. (2024) Nature structural & molecular biology 31, 874-883. PMID: 384591278. Polley, S. et al. (2024) Nature structural & molecular biology 31, 861-873. PMID: 38459128 |
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