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Anti-Human AK1/Adenylate kinase isoenzyme 1 Polyclonal Antibody (HF865014)

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规格:
  • 50ug
  • 100ug
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概述
货号HF865014
品牌abinScience
描述
Anti-Human AK1/Adenylate kinase isoenzyme 1 Polyclonal Antibody (HF865014) is a rabbit polyclonal antibody detecting AK1/Adenylate kinase isoenzyme 1 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, Human, Sus scrofa, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human AK1/Adenylate kinase isoenzyme 1 (Met1-Lys194).
靶标AK1/Adenylate kinase isoenzyme 1
纯化方式Purified by antigen affinity column.
Accession号P00568
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名Adenylate kinase isoenzyme 1, AK 1, 2.7.4.3, 2.7.4.6, ATP-AMP transphosphorylase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, Myokinase, AK1
背景

Adenylate kinase isoenzyme 1 (AK1) is a ~21 kDa protein. Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Also catalyzes at a very low rate the synthesis of thiamine triphosphate (ThTP) from thiamine diphosphate (ThDP) and ADP.

1. Panayiotou, C. et al. (2011) The Biochemical journal 433, 527-34. PMID: 21080915
2. Amiri, M. et al. (2013) The international journal of biochemistry & cell biology 45, 925-31. PMID: 23416111
3. Matsuura, S. et al. (1989) The Journal of biological chemistry 264, 10148-55. PMID: 2542324
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-AK1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse heart lysate
    Lane 2: Mouse skeletal muscle lysate
    Lane 3: Rat heart lysate
    Lane 4: Rat skeletal muscle lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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