Anti-Human D2HGDH Polyclonal Antibody (HV254014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HV254014

品牌

abinScience

描述

Anti-Human D2HGDH Polyclonal Antibody (HV254014) is a rabbit polyclonal antibody detecting D2HGDH in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human D2HGDH (Arg56-Asp232).

靶标

D2HGDH

纯化方式

Purified by antigen affinity column.

Accession号

Q8N465

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

别名

D-2-hydroxyglutarate dehydrogenase, mitochondrial, 1.1.99.39, D2HGDH, D2HGD

背景

D-2-hydroxyglutarate dehydrogenase, mitochondrial (D2HGDH) is a ~56 kDa protein. Catalyzes the oxidation of D-2-hydroxyglutarate (D-2-HG) to alpha-ketoglutarate. Also catalyzes the oxidation of other D-2-hydroxyacids, such as D-malate (D-MAL) and D-lactate (D-LAC). Exhibits high activities towards D-2-HG and D-MAL but a very weak activity towards D-LAC.

1. Achouri, Y. et al. (2004) The Biochemical journal 381, 35-42. PMID: 15070399

2. Struys, EA. et al. (2005) American journal of human genetics 76, 358-60. PMID: 15609246

3. Struys, EA. et al. (2005) Annals of neurology 58, 626-30. PMID: 16037974

4. Kranendijk, M. et al. (2010) Human mutation 31, 279-83. PMID: 20020533

5. Yang, J. et al. (2021) Cell discovery 7, 3. PMID: 33431826

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-D2HGDH polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate

参考文献

产品说明书

Anti-Human D2HGDH Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human D2HGDH Polyclonal Antibody (HV254014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human D2HGDH Polyclonal Antibody (HV254014)

概述

图片

参考文献

产品说明书

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