Anti-Mouse CASP4/Caspase-4 Polyclonal Antibody (MW474014) is a rabbit polyclonal antibody detecting CASP4/Caspase-4 in ELISA, IHC, WB. Suitable for Mus musculus.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
种属反应性
Mouse
应用
ELISA, IHC, WB
宿主
Rabbit
克隆类型
Polyclonal
同种型
IgG
免疫原
E. coli - derived recombinant Mouse CASP4/Caspase-4 (Pro81-Glu266).
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Caspase-4 (CASP4) is a ~42 kDa protein. Inflammatory caspase that acts as the effector of the non-canonical inflammasome by mediating lipopolysaccharide (LPS)-induced pyroptosis. Also indirectly activates the NLRP3 and NLRP6 inflammasomes. Acts as a thiol protease that cleaves a tetrapeptide after an Asp residue at position P1: catalyzes cleavage of CGAS and GSDMD. In contrast to its human ortholog, does not cleave IL18. Effector of the non-canonical inflammasome independently of NLRP3 inflammasome and CASP1: the non-canonical inflammasome promotes pyroptosis through GSDMD cleavage without involving secretion of cytokine IL1B and IL18.
1. Kayagaki, N. et al. (2011) Nature 479, 117-21. PMID: 22002608
2. Aachoui, Y. et al. (2013) Science (New York, N.Y.) 339, 975-8. PMID: 23348507
3. Kayagaki, N. et al. (2013) Science (New York, N.Y.) 341, 1246-9. PMID: 23887873
4. Hagar, JA. et al. (2013) Science (New York, N.Y.) 341, 1250-3. PMID: 24031018
5. Shi, J. et al. (2014) Nature 514, 187-92. PMID: 25119034
6. Lee, BL. et al. (2018) The Journal of experimental medicine 215, 2279-2288. PMID: 30135078
7. Zhu, F. et al. (2023) Immunity 56, 753-767.e8. PMID: 37001519
8. Wei, C. et al. (2024) Nature 629, 893-900. PMID: 38632402
9. Aachoui, Y. et al. (2015) Cell host & microbe 18, 320-32. PMID: 26320999
10. Hara, H. et al. (2018) Cell 175, 1651-1664.e14. PMID: 30392956
Note
For research use only.
图片
Western blot
Western blot analysis was performed using anti-CASP4 polyclonal antibody at 1ug/mL on various samples. Lane 1: U2OS cell lysate
