Anti-Human ACTR3 Polyclonal Antibody (HX211014)

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50ug
100ug

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概述
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参考文献
产品说明书

概述

货号

HX211014

品牌

abinScience

描述

Anti-Human ACTR3 Polyclonal Antibody (HX211014) is a rabbit polyclonal antibody detecting ACTR3 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, Human, Xenopus laevis, and Takifugu rubripes.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human ACTR3 (Pro6-Lys254).

靶标

ACTR3

纯化方式

Purified by antigen affinity column.

Accession号

P61158

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

别名

Actin-related protein 3, Actin-like protein 3, ACTR3, ARP3

背景

Actin-related protein 3 (ACTR3) is a ~47 kDa protein. ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA.

1. Welch, MD. et al. (1997) Nature 385, 265-9. PMID: 9000076

2. Rao, J. et al. (2017) The Journal of clinical investigation 127, 4257-4269. PMID: 29058690

3. Yoo, Y. et al. (2007) The Journal of biological chemistry 282, 7616-23. PMID: 17220302

4. Schrank, BR. et al. (2018) Nature 559, 61-66. PMID: 29925947

5. Kim, J. et al. (2010) Nature 464, 1048-51. PMID: 20393563

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-ACTR3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: A549 cell lysate
Lane 2: A431 cell lysate
Lane 3: HL60 cell lysate
Lane 4: Hela cell lysate
Lane 5: Jurkat cell lysate
Lane 6: K562 cell lysate
Lane 7: PC-3 cell lysate
Lane 8: U2OS cell lysate
Lane 9: Mouse spleen lysate
Lane 10: Mouse lung lysate
Lane11: Mouse liver lysate
Lane12: Rat spleen lysate
Lane13: Rat lung lysate
Lane14: Rat liver lysate

参考文献

产品说明书

Anti-Human ACTR3 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ACTR3 Polyclonal Antibody (HX211014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ACTR3 Polyclonal Antibody (HX211014)

概述

图片

参考文献

产品说明书

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