Anti-Human LMNA Polyclonal Antibody (HA509014) is a rabbit polyclonal antibody detecting LMNA in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, Human, Sus scrofa, and Gallus gallus.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
种属反应性
Human, Mouse, Rat
应用
ELISA, IHC, WB
宿主
Rabbit
克隆类型
Polyclonal
同种型
IgG
免疫原
E. coli - derived recombinant Human LMNA (Thr27-Ile229).
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Prelamin-A/C (LMNA) is a ~74 kDa protein. Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Lamin A and C also regulate matrix stiffness by conferring nuclear mechanical properties. The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively. Lamin A and C are present in equal amounts in the lamina of mammals.
1. Bonne, G. et al. (1999) Nature genetics 21, 285-8. PMID: 10080180
2. Fatkin, D. et al. (1999) The New England journal of medicine 341, 1715-24. PMID: 10580070
3. Cao, H. et al. (2000) Human molecular genetics 9, 109-12. PMID: 10587585
4. Muchir, A. et al. (2000) Human molecular genetics 9, 1453-9. PMID: 10814726
5. De Sandre-Giovannoli, A. et al. (2002) American journal of human genetics 70, 726-36. PMID: 11799477
6. Novelli, G. et al. (2002) American journal of human genetics 71, 426-31. PMID: 12075506
7. Chen, L. et al. (2003) Lancet (London, England) 362, 440-5. PMID: 12927431
8. Navarro, CL. et al. (2004) Human molecular genetics 13, 2493-503. PMID: 15317753
9. Quijano-Roy, S. et al. (2008) Annals of neurology 64, 177-86. PMID: 18551513
10. Renou, L. et al. (2008) Journal of medical genetics 45, 666-71. PMID: 18611980
Note
For research use only.
图片
Western blot
Western blot analysis was performed using anti-LMNA polyclonal antibody at 1ug/mL on various samples. Lane 1: A549 cell lysate Lane 2: Hela cell lysate Lane 3: A375 cell lysate
