Anti-Human TRIB3 Polyclonal Antibody (HA514014)

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规格：

50ug
100ug

数量：

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概述
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参考文献
产品说明书

概述

货号

HA514014

品牌

abinScience

描述

Anti-Human TRIB3 Polyclonal Antibody (HA514014) is a rabbit polyclonal antibody detecting TRIB3 in ELISA, IHC, WB. Suitable for Bovine and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human TRIB3 (Ser66-Gln317).

靶标

TRIB3

纯化方式

Purified by antigen affinity column.

Accession号

Q96RU7

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

别名

Tribbles homolog 3, TRB-3, Neuronal cell death-inducible putative kinase, SINK, p65-interacting inhibitor of NF-kappa-B, TRIB3, C20orf97, NIPK, SKIP3, TRB3

背景

Tribbles homolog 3 (TRIB3) is a ~39 kDa protein. Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR: while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity. Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation.

1. Ohoka, N. et al. (2005) The EMBO journal 24, 1243-55. PMID: 15775988

2. Ord, D. et al. (2005) Biochemical and biophysical research communications 330, 210-8. PMID: 15781252

3. Wu, M. et al. (2003) The Journal of biological chemistry 278, 27072-9. PMID: 12736262

4. Kiss-Toth, E. et al. (2004) The Journal of biological chemistry 279, 42703-8. PMID: 15299019

5. Aynaud, MM. et al. (2012) The Journal of biological chemistry 287, 39182-92. PMID: 22977230

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-TRIB3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: LO2 cell lysate
Lane 2: PC-3 cell lysate

参考文献

产品说明书

Anti-Human TRIB3 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human TRIB3 Polyclonal Antibody (HA514014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human TRIB3 Polyclonal Antibody (HA514014)

概述

图片

参考文献

产品说明书

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