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Anti-Human SIX1 Polyclonal Antibody (HA515014)

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规格:
  • 50ug
  • 100ug
数量:
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  • 参考文献

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概述
货号HA515014
品牌abinScience
描述
Anti-Human SIX1 Polyclonal Antibody (HA515014) is a rabbit polyclonal antibody detecting SIX1 in ELISA, IHC, WB. Suitable for Mus musculus, Danio rerio, Human, and Xenopus laevis.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Human, Mouse
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human SIX1 (Met1-Lys199).
靶标SIX1
纯化方式Purified by antigen affinity column.
Accession号Q15475
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX1
背景

Homeobox protein SIX1 is a ~32 kDa protein. Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development. Plays an important role in the development of several organs, including kidney, muscle and inner ear. Depending on context, functions as a transcriptional repressor or activator. Lacks an activation domain, and requires interaction with EYA family members for transcription activation. Mediates nuclear translocation of EYA1 and EYA2.

1. Ruf, RG. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 8090-5. PMID: 15141091
2. Patrick, AN. et al. (2009) The Journal of biological chemistry 284, 20781-90. PMID: 19497856
3. Patrick, AN. et al. (2013) Nature structural & molecular biology 20, 447-53. PMID: 23435380
4. Brunmeir, R. et al. (2016) PLoS genetics 12, e1006474. PMID: 27923061
5. Coletta, RD. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 6478-83. PMID: 15123840
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-SIX1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse skeletal muscle lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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