Anti-Human LATS1 Polyclonal Antibody (HA517014)

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50ug
100ug

数量：

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概述
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参考文献
产品说明书

概述

货号

HA517014

品牌

abinScience

描述

Anti-Human LATS1 Polyclonal Antibody (HA517014) is a rabbit polyclonal antibody detecting LATS1 in ELISA, IHC, WB. Suitable for Mus musculus and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human LATS1 (Ser635-Tyr1076).

靶标

LATS1

纯化方式

Purified by antigen affinity column.

Accession号

O95835

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

别名

Serine/threonine-protein kinase LATS1, 2.7.11.1, Large tumor suppressor homolog 1, WARTS protein kinase, h-warts, LATS1, WARTS

背景

Serine/threonine-protein kinase LATS1 is a ~126 kDa protein. Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity.

1. Nishiyama, Y. et al. (1999) FEBS letters 459, 159-65. PMID: 10518011

2. Hirota, T. et al. (2000) The Journal of cell biology 149, 1073-86. PMID: 10831611

3. Hao, Y. et al. (2008) The Journal of biological chemistry 283, 5496-509. PMID: 18158288

4. Meng, Z. et al. (2015) Nature communications 6, 8357. PMID: 26437443

5. Britschgi, A. et al. (2017) Nature 541, 541-545. PMID: 28068668

10. Nie, L. et al. (2024) Molecular cell 84, 3336-3353.e7. PMID: 39173637

Note

For research use only.

图片

参考文献

产品说明书

Anti-Human LATS1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human LATS1 Polyclonal Antibody (HA517014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human LATS1 Polyclonal Antibody (HA517014)

概述

图片

参考文献

产品说明书

相关产品推荐

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