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Anti-Human HSPB11 Polyclonal Antibody (HA518014)

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规格:
  • 50ug
  • 100ug
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  • 参考文献

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概述
货号HA518014
品牌abinScience
描述
Anti-Human HSPB11 Polyclonal Antibody (HA518014) is a rabbit polyclonal antibody detecting HSPB11 in ELISA, IHC, WB. Suitable for Mus musculus and Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human HSPB11 (Met1-Ser144).
靶标HSPB11
纯化方式Purified by antigen affinity column.
Accession号Q9Y547
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名Intraflagellar transport protein 25 homolog, Heat shock protein beta-11, Hspb11, Placental protein 25, PP25, HSPB11, C1orf41, IFT25, HSPC034
背景

Intraflagellar transport protein 25 homolog (HSPB11) is a ~16 kDa protein. Component of the IFT complex B required for sonic hedgehog/SHH signaling. May mediate transport of SHH components: required for the export of SMO and PTCH1 receptors out of the cilium and the accumulation of GLI2 at the ciliary tip in response to activation of the SHH pathway, suggesting it is involved in the dynamic transport of SHH signaling molecules within the cilium. Not required for ciliary assembly. Its role in intraflagellar transport is mainly seen in tissues rich in ciliated cells such as kidney and testis. Essential for male fertility, spermiogenesis and sperm flagella formation.

NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-HSPB11 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: A549 cell lysate
    Lane 3: Lncap cell lysate
    Lane 5: K562 cell lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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