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Anti-Human jo-1 Polyclonal Antibody (HY584024)

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规格:
  • 50ug
  • 100ug
数量:
  • 概述

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  • 参考文献

  • 产品说明书

概述
货号HY584024
品牌abinScience
描述
Anti-Human jo-1 Polyclonal Antibody (HY584024) is a rabbit polyclonal antibody detecting jo-1 in ELISA, IHC, WB. Suitable for Mus musculus, Human, Pongo abelii, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
种属反应性Human, Mouse
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human jo-1 (Met1-Cys509).
靶标jo-1
纯化方式Purified by antigen affinity column.
Accession号P12081
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名Histidine--tRNA ligase, cytoplasmic, 6.1.1.21, Histidyl-tRNA synthetase, HisRS, HARS1, HARS, HRS
背景

Histidine--tRNA ligase, cytoplasmic (jo-1) is a ~57 kDa protein. Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). Plays a role in axon guidance.

1. Abbott, JA. et al. (2018) Human mutation 39, 415-432. PMID: 29235198
2. Safka Brozkova, D. et al. (2015) Brain : a journal of neurology 138, 2161-72. PMID: 26072516
NoteFor research use only.
图片
  • Western blot

    Western blot analysis was performed using anti-HARS1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: 293T cell lysate
    Lane 3: K562 cell lysate
    Lane 4: HepG2 cell lysate
    Lane 5: HCT116 cell lysate
    Lane 6: Jurkat cell lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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