Anti-Human CUL1 Polyclonal Antibody (HC489014)

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50ug
100ug

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概述
图片
参考文献
产品说明书

概述

货号

HC489014

品牌

abinScience

描述

Anti-Human CUL1 Polyclonal Antibody (HC489014) is a rabbit polyclonal antibody detecting CUL1 in ELISA, IHC, WB. Suitable for Mus musculus and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human CUL1 (Met1-Glu250).

靶标

CUL1

纯化方式

Purified by antigen affinity column.

Accession号

Q13616

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

别名

Cullin-1, CUL-1, CUL1

背景

Cullin-1 (CUL1) is a ~89 kDa protein. Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1.

1. Gao, D. et al. (2011) Molecular cell 44, 290-303. PMID: 22017875

2. Duan, S. et al. (2011) Molecular cell 44, 317-24. PMID: 22017877

3. Scott, DC. et al. (2016) Cell 166, 1198-1214.e24. PMID: 27565346

4. Liwocha, J. et al. (2024) Nature structural & molecular biology 31, 378-389. PMID: 38326650

5. Min, KW. et al. (2003) The Journal of biological chemistry 278, 15905-10. PMID: 12609982

6. Pagan, JK. et al. (2015) Nature cell biology 17, 31-43. PMID: 25503564

7. Man, X. et al. (2015) European journal of cell biology 94, 162-72. PMID: 25704143

8. Hopf, LVM. et al. (2022) Nature structural & molecular biology 29, 854-862. PMID: 35982156

10. Muhar, MF. et al. (2025) Nature 638, 519-527. PMID: 39880951

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-CUL1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate
Lane 2: Rat brain lysate

参考文献

产品说明书

Anti-Human CUL1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CUL1 Polyclonal Antibody (HC489014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CUL1 Polyclonal Antibody (HC489014)

概述

图片

参考文献

产品说明书

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