Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
5-hydroxytryptamine receptor 2A (HTR2A) is a ~52 kDa protein. G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. HTR2A is coupled to G(q)/G(11) G alpha proteins and activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers that modulate the activity of phosphatidylinositol 3-kinase and promote the release of Ca(2+) ions from intracellular stores, respectively. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways.
1. Stam, NJ. et al. (1992) European journal of pharmacology 227, 153-62. PMID: 1330647
2. Cussac, D. et al. (2008) European journal of pharmacology 594, 32-8. PMID: 18703043
3. Knauer, CS. et al. (2009) Naunyn-Schmiedeberg's archives of pharmacology 379, 461-71. PMID: 19057895
4. Albizu, L. et al. (2011) Neuropharmacology 61, 770-7. PMID: 21645528
5. Delille, HK. et al. (2012) Neuropharmacology 62, 2184-91. PMID: 22300836
6. Cao, D. et al. (2022) Science (New York, N.Y.) 375, 403-411. PMID: 35084960
7. Chen, Z. et al. (2024) Cell 187, 2194-2208.e22. PMID: 38552625
8. Wacker, D. et al. (2017) Cell 168, 377-389.e12. PMID: 28129538
9. González-Maeso, J. et al. (2008) Nature 452, 93-7. PMID: 18297054
Note
For research use only.
图片
Western blot
Western blot analysis was performed using anti-HTR2A polyclonal antibody at 1ug/mL on various samples. Lane 1: Mouse brain lysate Lane 2: Mouse cerebellum lysate Lane 3: Rat brain lysate Lane 4: Rat cerebellum lysate
