Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Bifunctional glutamate/proline--tRNA ligase (EPRS1) is a ~170 kDa protein. Multifunctional protein which primarily functions within the aminoacyl-tRNA synthetase multienzyme complex, also known as multisynthetase complex. Within the complex it catalyzes the attachment of both L-glutamate and L-proline to their cognate tRNAs in a two-step reaction where the amino acid is first activated by ATP to form a covalent intermediate with AMP. Subsequently, the activated amino acid is transferred to the acceptor end of the cognate tRNA to form L-glutamyl-tRNA(Glu) and L-prolyl-tRNA(Pro). Upon interferon-gamma stimulation, EPRS1 undergoes phosphorylation, causing its dissociation from the aminoacyl-tRNA synthetase multienzyme complex. It is recruited to form the GAIT complex, which binds to stem loop-containing GAIT elements found in the 3'-UTR of various inflammatory mRNAs, such as ceruloplasmin.
1. Zhou, H. et al. (2013) Nature 494, 121-4. PMID: 23263184
2. Son, J. et al. (2013) Acta crystallographica. Section D, Biological crystallography 69, 2136-45. PMID: 24100331
3. Mendes, MI. et al. (2018) American journal of human genetics 102, 676-684. PMID: 29576217
4. Thömmes, P. et al. (1988) Nucleic acids research 16, 5391-406. PMID: 3290852
5. Yoon, I. et al. (2023) EMBO molecular medicine 15, e16940. PMID: 37212275
6. Sampath, P. et al. (2004) Cell 119, 195-208. PMID: 15479637
7. Arif, A. et al. (2012) Molecular and cellular biology 32, 5046-55. PMID: 23071094
8. Arif, A. et al. (2017) Nature 542, 357-361. PMID: 28178239
