Affinity Purified — Minimal background and high purity for reliable results.
Multi-Application — Validated across multiple applications.
Multi-Species — Cross-reactive for translational research.
种属反应性
Human, Mouse, Rat
应用
ELISA, IHC, WB
宿主
Rabbit
同种型
IgG
克隆类型
Polyclonal
免疫原
E. coli - derived recombinant Human MLST8 (Tyr68-Trp247).
靶标
GBL,TORC subunit LST8,Mammalian lethal with SEC13 protein 8,G protein beta subunit-like,Gable,Protein GbetaL,mLST8,LST8,MLST8,Target of rapamycin complex subunit LST8
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Target of rapamycin complex subunit LST8 (MLST8) is a ~35 kDa protein. Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. In response to nutrients, mTORC1 is recruited to the lysosome membrane and promotes protein, lipid and nucleotide synthesis by phosphorylating several substrates, such as ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1). In the same time, it inhibits catabolic pathways by phosphorylating the autophagy initiation components ULK1 and ATG13, as well as transcription factor TFEB, a master regulators of lysosomal biogenesis and autophagy. The mTORC1 complex is inhibited in response to starvation and amino acid depletion.
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5. Wang, B. et al. (2017) Nature 545, 365-369. PMID: 28489822
6. Yu, Z. et al. (2022) The Journal of biological chemistry 298, 102288. PMID: 35926713
