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Anti-TOP2A Polyclonal Antibody (PTX20172)

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概述
货号PTX20172
品牌ProteoGenix
描述
Anti-TOP2A Polyclonal Antibody (PTX20172) is a rabbit polyclonal antibody detecting DNA topoisomerase II, alpha isozyme, TOP2A, DNA topoisomerase 2-alpha, TOP2 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human TOP2A (Ile923-Arg1148).
靶标DNA topoisomerase II, alpha isozyme, TOP2A, DNA topoisomerase 2-alpha, TOP2
纯化方式Purified by antigen affinity column.
Accession号P11388
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

DNA topoisomerase 2-alpha (TOP2A) is a ~174 kDa protein. Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. May play a role in regulating the period length of BMAL1 transcriptional oscillation.

1. Wyles, JP. et al. (2007) Nucleic acids research 35, 4076-85. PMID: 17567603
2. Williamson, EA. et al. (2008) Nucleic acids research 36, 5822-31. PMID: 18790802
3. Ramamoorthy, M. et al. (2012) Nucleic acids research 40, 1621-35. PMID: 22013166
4. Lee, S. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 2925-30. PMID: 22323612
5. Kratz, AS. et al. (2016) Cell cycle (Georgetown, Tex.) 15, 3419-3431. PMID: 27754753
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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