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Anti-Human BUB1 Polyclonal Antibody (PTX20474)

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概述
货号PTX20474
品牌ProteoGenix
描述
Anti-Human BUB1 Polyclonal Antibody (PTX20474) is a rabbit polyclonal antibody detecting BUB1L, hBUB1, BUB1A, Mitotic checkpoint serine/threonine-protein kinase BUB1, BUB1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human BUB1 (Met1-His151).
靶标BUB1L, hBUB1, BUB1A, Mitotic checkpoint serine/threonine-protein kinase BUB1, BUB1
纯化方式Purified by antigen affinity column.
Accession号O43683
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Mitotic checkpoint serine/threonine-protein kinase BUB1 is a ~122 kDa protein. Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion.

1. Carvalhal, S. et al. (2022) Science advances 8, eabk0114. PMID: 35044816
NoteFor research use only.
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参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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