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Anti-LIPE Polyclonal Antibody (PTX20517)

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概述
货号PTX20517
品牌ProteoGenix
描述
Anti-LIPE Polyclonal Antibody (PTX20517) is a rabbit polyclonal antibody detecting Monoacylglycerol lipase LIPE, LIPE, HSL, Retinyl ester hydrolase, REH, Hormone-sensitive lipase in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Human, Mouse
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human LIPE (Met302-Leu425).
靶标Monoacylglycerol lipase LIPE, LIPE, HSL, Retinyl ester hydrolase, REH, Hormone-sensitive lipase
纯化方式Purified by antigen affinity column.
Accession号Q05469
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Hormone-sensitive lipase (LIPE) is a ~116 kDa protein. Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters. Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs. Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs. Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.

1. Ali, YB. et al. (2005) Journal of lipid research 46, 994-1000. PMID: 15716583
2. Gao, J. et al. (2005) The Journal of investigative dermatology 124, 1259-66. PMID: 15955102
3. Rodriguez, JA. et al. (2010) Biochimica et biophysica acta 1801, 77-83. PMID: 19800417
4. Holst, LS. et al. (1996) Genomics 35, 441-7. PMID: 8812477
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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