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Anti-CHIKV Spike glycoprotein E2 Antibody (5F10) (PTX19140)

概述
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参考文献

概述

货号	PTX19140
品牌	ProteoGenix
描述	Anti-CHIKV Spike glycoprotein E2 Antibody (5F10) (PTX19140) Purity: >95% as determined by SDS-PAGE.. Highlights Protein A/G purified from cell culture supernatant. — Ensuring high specificity and minimal background. Multi-Application — Validated across multiple applications. Multi-Species — Cross-reactive for translational research.
种属反应性	Chikungunya virus (strain S27-African prototype) (CHIKV), Chikungunya virus (strain Nagpur) (CHIKV), Chikungunya virus (strain 37997) (CHIKV)
应用	ELISA, IF, WB
宿主	Human
同种型	IgG1, kappa
克隆号	5F10
克隆类型	Monoclonal
靶标	Structural polyprotein, Envelope glycoprotein E2, Spike glycoprotein E2
内毒素水平	Please contact with the lab for this information.
纯度	>95% as determined by SDS-PAGE.
纯化方式	Protein A/G purified from cell culture supernatant.
Accession号	Q8JUX5, Q5XXP3, Q5WQY5
状态	Liquid
保存溶液	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Structural polyprotein (Spike glycoprotein E2) is a ~138 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions. 1. Webb, LG. et al. (2020) PLoS pathogens 16, e1008999. PMID: 33057424
Note	For research use only.

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参考文献

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

=

浓度

×

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

×

V₁ (起始体积)

=

工作液

C₂ (终浓度)

×

V₂ (终体积)

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Anti-CHIKV Spike glycoprotein E2 Antibody (5F10)

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