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Anti-Murine minute virus/MVM NS1 Polyclonal Antibody (VK011034)

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概述
货号VK011034
品牌abinScience
描述
Anti-Murine minute virus/MVM NS1 Polyclonal Antibody (VK011034) is a rabbit polyclonal antibody detecting NS1 in ELISA, IHC, WB. Suitable for Murine minute virus and Parvovirus LuIII.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
种属反应性Murine minute virus (MVM)
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Murine minute virus NS1 (Tyr6-Thr253).
靶标NS1
纯度>90%
纯化方式Purified by antigen affinity column.
Accession号P03134
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名Initiator protein NS1, NS1, EC:3.1.21.-, EC:3.6.4.12, NCVP1, Non-capsid protein NS-1, Non-structural protein NS1, NS1
背景

Initiator protein NS1 is a ~76 kDa protein. Multifunctional protein which displays endonuclease and helicase activities required for initiating and directing viral DNA replication. Also plays a role in viral packaging and transactivation of several promoters. Binds site-specifically to 2-3 approximate tandem copies of the tetranucleotide 5'TGGT3' within the origins of replication (Ori), unwinds this hairpin region and nicks one DNA strand thereby initiating the rolling circle replication (RCR). Cooperatively binds Ori with host PIF and probably other host factors, which activate the nickase function of NS1. Becomes covalently attached to the 5' end of the nick and provides a 3'OH for priming DNA synthesis.

1. Christensen, J. et al. (2002) Journal of virology 76, 6518-31. PMID: 12050365
2. Christensen, J. et al. (1995) Journal of virology 69, 5422-30. PMID: 7636987
3. Cotmore, SF. et al. (2007) Journal of virology 81, 13015-27. PMID: 17898054
4. Willwand, K. et al. (1997) The Journal of general virology 78 ( Pt 10), 2647-55. PMID: 9349487
5. Christensen, J. et al. (2001) Journal of virology 75, 7009-17. PMID: 11435581
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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