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Anti Arabidopsis HSP70 Polyclonal Antibody (PR002014)

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概述
货号PR002014
品牌abinScience
描述
Anti Arabidopsis HSP70 Polyclonal Antibody (PR002014) is a rabbit polyclonal antibody detecting HSP70 in WB, IHC, ELISA. Suitable for Glycine max, Petunia hybrida, Spinacia oleracea, and Zea mays.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Arabidopsis thaliana (Mouse-ear cress), Glycine max (Soybean) (Glycine hispida), Solanum lycopersicum (Tomato)
应用WB, IHC, ELISA
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Arabidopsis HSP70-3 (Met1-Asn391).
靶标HSP70
内毒素水平Please contact with the lab for this information.
纯度>90% as determined by SDS-PAGE.
纯化方式Purified by antigen affinity column.
Accession号O65719
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名Heat shock 70 kDa protein 3, AtHsp70-3, Heat shock protein 70-3, Heat shock cognate 70 kDa protein 3, AtHsc70-3, Heat shock cognate protein 70-3, HSP70-3
背景

Heat shock 70 kDa protein 3 (HSP70) is a ~71 kDa protein. In cooperation with other chaperones, Hsp70s are key components that facilitate folding of de novo synthesized proteins, assist translocation of precursor proteins into organelles, and are responsible for degradation of damaged protein under stress conditions.

NoteFor research use only.
图片
  • Western blot

    Lane 1: Hela cell lysate
    Lane 2: A431 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: 293T cell lysate
    Lane 5: K562 cell lysate
    Lane 6: NIH3T3 cell lysate
    Lane 7: Mouse brain lysate
    Lane 8: Rat brain lysate
    Lane 9: Rat liver lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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