Anti-PDIA3 Polyclonal Antibody (HB133014) is a rabbit polyclonal antibody detecting PDIA3 in WB, IHC, ELISA. Suitable for Human, Mus musculus, Rattus norvegicus, and Bovine.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
种属反应性
Human
应用
WB, IHC, ELISA
宿主
Rabbit
克隆类型
Polyclonal
同种型
IgG
免疫原
E. coli - derived recombinant PDIA3 (Asp162-Tyr416).
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
Protein disulfide-isomerase A3, EC:5.3.4.1, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, p58, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, ER protein 57, ERp57, Endoplasmic reticulum resident protein 60, ER protein 60, ERp60, PDIA3
背景
Protein disulfide-isomerase A3 (PDIA3) is a ~56 kDa protein. Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone. Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it functions as an essential folding chaperone for TAPBP. Through TAPBP, assists the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Therefore, plays a crucial role in the presentation of antigens to cytotoxic T cells in adaptive immunity.
5. Bourdi, M. et al. (1995) Archives of biochemistry and biophysics 323, 397-403. PMID: 7487104
6. Domnick, A. et al. (2022) Nature communications 13, 4701. PMID: 35948544
Note
For research use only.
图片
Western blot
Western blot analysis was performed using anti-PDIA3 polyclonal antibody at 1ug/mL on various samples. Lane 1: HepG2 cell lysate Lane 2: A375 cell lysate Lane 3: LO2 cell lysate Lane 4: U2OS cell lysate Lane 5: Lncap cell lysate Lane 6: K562 cell lysate Lane 7: Jurkat cell lysate
