Anti-Human NSMCE2 Polyclonal Antibody (HA545014)

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50ug
100ug

数量：

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概述
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参考文献
产品说明书

概述

货号

HA545014

品牌

abinScience

描述

Anti-Human NSMCE2 Polyclonal Antibody (HA545014) is a rabbit polyclonal antibody detecting NSMCE2 in WB, IHC, ELISA. Suitable for Human and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human

应用

WB, IHC, ELISA

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human NSMCE2 (Ser16-Glu247).

靶标

NSMCE2

内毒素水平

Please contact with the lab for this information.

纯度

>90% as determined by SDS-PAGE.

纯化方式

Purified by antigen affinity column.

Accession号

Q96MF7

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

别名

E3 SUMO-protein ligase NSE2, EC:2.3.2.-, E3 SUMO-protein transferase NSE2, MMS21 homolog, hMMS21, Non-structural maintenance of chromosomes element 2 homolog, Non-SMC element 2 homolog, NSMCE2

背景

E3 SUMO-protein ligase NSE2 (NSMCE2) is a ~27 kDa protein. E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. Is not be required for the stability of the complex. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Acts as an E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TSNAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, RAD51AP1, and maybe the cohesin components RAD21 and STAG2.

1. Potts, PR. et al. (2005) Molecular and cellular biology 25, 7021-32. PMID: 16055714

2. Potts, PR. et al. (2006) The EMBO journal 25, 3377-88. PMID: 16810316

3. Potts, PR. et al. (2007) Nature structural & molecular biology 14, 581-90. PMID: 17589526

Note

For research use only.

图片

Western blot

Western blot analysis was performed using anti-NSMCE2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate
Lane 2: A549 cell lysate
Lane 3: 293T cell lysate

参考文献

产品说明书

Anti-Human NSMCE2 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human NSMCE2 Polyclonal Antibody (HA545014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human NSMCE2 Polyclonal Antibody (HA545014)

概述

图片

参考文献

产品说明书

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