Anti-PDHA2 Polyclonal Antibody (HA574014)

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50ug
100ug

数量：

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概述
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参考文献
产品说明书

概述

货号

HA574014

品牌

abinScience

描述

Anti-PDHA2 Polyclonal Antibody (HA574014) is a rabbit polyclonal antibody detecting PDHA2 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, Rat, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human PDHA2 (Ser28-Ser388).

靶标

Pyruvate dehydrogenase E1 component subunit alpha testis-specific form mitochondrial, EC:1.2.4.1, PDHE1-A type II, PDHA2

内毒素水平

Please contact with the lab for this information.

观测分子量

~43 kDa

纯化方式

Purified by antigen affinity column.

Accession号

P29803, P35486, , A7MB35, P26284, P29804, P08559

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial (PDHA2) is a ~42 kDa protein. Together with PDHB forms the heterotetrameric E1 subunit of the pyruvate dehydrogenase (PDH) complex in testis. The PDH complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle (Probable). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3) (Probable). The E1 subunit catalyzes both the thiamine pyrophosphate (TPP)-dependent decarboxylation of pyruvate and the reductive acetylation of a lipoyl group covalently linked to the lipoyl-bearing domains of E2.

1. Hiromasa, Y. et al. (2004) The Journal of biological chemistry 279, 6921-33. PMID: 14638692

2. Korotchkina, LG. et al. (2006) The Journal of biological chemistry 281, 9688-96. PMID: 16436377

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-PDHA2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate
Lane 2: Mouse kidney lysate
Lane 3: Mouse testis lysate
Lane 4: Rat brain lysate
Lane 5: Rat kidney lysate

参考文献

产品说明书

Anti-PDHA2 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PDHA2 Polyclonal Antibody (HA574014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-PDHA2 Polyclonal Antibody (HA574014)

概述

图片

参考文献

产品说明书

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