Anti-Semliki forest virus/SFV Capsid Polyclonal Antibody (VK022014) is a rabbit polyclonal antibody detecting Capsid protein in WB, IHC, ELISA. Suitable for O'nyong-nyong virus, Sagiyama virus, Chikungunya virus, Ross river virus, and Middelburg virus.
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
种属反应性
Semliki Forest virus (SFV), O'nyong'nyong virus (ONNV), Chikungunya virus (CHIKV), Ross River virus (RRV), Getah virus (GETV), Mayaro virus (MAYV)
应用
WB,IHC,ELISA
宿主
Rabbit
克隆类型
Polyclonal
同种型
IgG
免疫原
E. coli - derived recombinant Semliki forest virus/SFV Capsid (Cys119-Trp267).
靶标
Structural polyprotein, p130, Capsid protein, EC:3.4.21.90, Coat protein, C
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Structural polyprotein (Capsid) is a ~138 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.
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2. Melancon, P. et al. (1987) Journal of virology 61, 1301-9. PMID: 3553612
3. Skoging, U. et al. (1998) Journal of molecular biology 279, 865-72. PMID: 9642067
4. Ulmanen, I. et al. (1979) Virology 99, 265-76. PMID: 516447
5. Vonderheit, A. et al. (2005) PLoS biology 3, e233. PMID: 15954801
6. Singh, I. et al. (1992) Journal of virology 66, 7049-58. PMID: 1433506
Note
For research use only.
图片
Western blot
Lane 1: recombinant Semliki forest virus/SFV Capsid (Catalog No: VK022012)
