Anti-GPR183 Polyclonal Antibody (HB793014)

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产品说明书

概述

货号

HB793014

品牌

abinScience

描述

Anti-GPR183 Polyclonal Antibody (HB793014) is a rabbit polyclonal antibody detecting G-protein coupled receptor 183; Epstein-Barr virus-induced G-protein coupled receptor 2; EBI2; EBV-induced G-protein coupled receptor 2; hEBI2; GPR183; EBI2 in ELISA, IHC, WB. Suitable for Human, Bovine, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human GPR183 (Lys313-Lys361).

靶标

G-protein coupled receptor 183; Epstein-Barr virus-induced G-protein coupled receptor 2; EBI2; EBV-induced G-protein coupled receptor 2; hEBI2; GPR183; EBI2

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P32249, Q1RMI1, Q3U6B2, D4A7K7

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

G-protein coupled receptor 183 (GPR183) is a ~41 kDa protein. G-protein coupled receptor expressed in lymphocytes that acts as a chemotactic receptor for B-cells, T-cells, splenic dendritic cells, monocytes/macrophages and astrocytes. Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols. Mediates cell positioning and movement of a number of cells by binding the 7-alpha,25-OHC ligand that forms a chemotactic gradient. Binding of 7-alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses. Guides B-cell movement along the B-cell zone-T-cell zone boundary and later to interfollicular and outer follicular regions.

1. Hannedouche, S. et al. (2011) Nature 475, 524-7. PMID: 21796212

2. Benned-Jensen, T. et al. (2012) The Journal of biological chemistry 287, 35470-35483. PMID: 22875855

3. Zhang, L. et al. (2012) Molecular pharmacology 82, 1094-103. PMID: 22930711

4. Barroso, R. et al. (2012) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26, 4841-54. PMID: 22913878

5. Rutkowska, A. et al. (2015) Glia 63, 341-51. PMID: 25297897

6. Benned-Jensen, T. et al. (2011) The Journal of biological chemistry 286, 29292-29302. PMID: 21673108

Note

For research use only

图片

参考文献

产品说明书

Anti-GPR183 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

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Anti-GPR183 Polyclonal Antibody (HB793014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-GPR183 Polyclonal Antibody (HB793014)

概述

图片

参考文献

产品说明书

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