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Anti-GTF2F1 Polyclonal Antibody (HA622014)

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规格:
  • 50ug
  • 100ug
数量:
  • 概述

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  • 参考文献

  • 产品说明书

概述
货号HA622014
品牌abinScience
描述
Anti-GTF2F1 Polyclonal Antibody (HA622014) is a rabbit polyclonal antibody detecting General transcription factor IIF subunit 1; General transcription factor IIF 74 kDa subunit; Transcription initiation factor IIF subunit alpha; TFIIF-alpha; Transcription initiation factor RAP74; GTF2F1; RAP74 in ELISA, IHC, WB. Suitable for Human, Bovine, Rat, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
种属反应性Human, Mouse, Rat
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant human GTF2F1 (Met1-Asp190).
靶标General transcription factor IIF subunit 1; General transcription factor IIF 74 kDa subunit; Transcription initiation factor IIF subunit alpha; TFIIF-alpha; Transcription initiation factor RAP74; GTF2F1; RAP74
内毒素水平Please contact with the lab for this information.
纯化方式Purified by antigen affinity column.
Accession号P35269, Q5EA53, Q6AY96, Q3THK3
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

General transcription factor IIF subunit 1 (GTF2F1) is a ~58 kDa protein. TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.

NoteFor research use only
图片
  • Western blot

    Western blot analysis was performed using anti-GTF2F1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: K562 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: Hela cell lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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