Anti-NAA10 Polyclonal Antibody (HA630014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HA630014

品牌

abinScience

描述

Anti-NAA10 Polyclonal Antibody (HA630014) is a rabbit polyclonal antibody detecting N-alpha-acetyltransferase 10; 2.3.1.255; N-terminal acetyltransferase complex ARD1 subunit homolog A; hARD1; NatA catalytic subunit Naa10; NAA10; ARD1; ARD1A; TE2 in ELISA, IHC, WB. Suitable for Human, Bovine, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant human NAA10 (Met1-Ser235).

靶标

N-alpha-acetyltransferase 10; 2.3.1.255; N-terminal acetyltransferase complex ARD1 subunit homolog A; hARD1; NatA catalytic subunit Naa10; NAA10; ARD1; ARD1A; TE2

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P41227, Q2KI14, Q9QY36

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

N-alpha-acetyltransferase 10 (NAA10) is a ~26 kDa protein. Catalytic subunit of N-terminal acetyltransferase complexes which display alpha (N-terminal) acetyltransferase activity. Acetylates amino termini that are devoid of initiator methionine. The alpha (N-terminal) acetyltransferase activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration.

1. Arnesen, T. et al. (2005) The Biochemical journal 386, 433-43. PMID: 15496142

2. Arnesen, T. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 8157-62. PMID: 19420222

3. Shin, DH. et al. (2009) PloS one 4, e7451. PMID: 19826488

4. Kuo, HP. et al. (2010) Science signaling 3, ra9. PMID: 20145209

5. Arnesen, T. et al. (2010) Molecular and cellular biology 30, 1898-909. PMID: 20154145

6. Myklebust, LM. et al. (2015) Human molecular genetics 24, 1956-76. PMID: 25489052

7. Seo, JH. et al. (2016) Nature communications 7, 12882. PMID: 27708256

8. Gottlieb, L. et al. (2018) Structure (London, England : 1993) 26, 925-935.e8. PMID: 29754825

9. Deng, S. et al. (2020) Nature communications 11, 818. PMID: 32042062

10. Jeong, JW. et al. (2002) Cell 111, 709-20. PMID: 12464182

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-NAA10 polyclonal antibody at 1ug/mL on various samples.
Lane 1: 293T cell lysate
Lane 2: Hela cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: HT-29 cell lysate
Lane 5: C2C12 cell lysate
Lane 6: H1299 cell lysate

参考文献

产品说明书

Anti-NAA10 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-NAA10 Polyclonal Antibody (HA630014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-NAA10 Polyclonal Antibody (HA630014)

概述

图片

参考文献

产品说明书

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